Journal: Clinical and Translational Medicine

Article Title: MiRNA‐501‐3p and MiRNA‐502‐3p: A promising biomarker panel for Alzheimer's disease

doi: 10.1002/ctm2.70389

Figure Lengend Snippet: Cell type‐specific expression of miR‐501‐3p and miR‐502‐3p in human and mouse brain‐derived neurons, astrocytes and microglia cells. (A) Representative microscopy images of cortical neurons, microglia and astrocytes derived from human brain (B) Representative microscopy images of hippocampal neurons, microglia and astrocytes derived from mouse brain (40×, scale bars: 100 µm). (C) qRT‐PCR analysis of miR‐501‐3p relative expression in human cortical neurons, microglia and astrocytes (D) qRT‐PCR analysis of miR‐501‐3p relative expression in mouse hippocampal neurons, microglia and astrocytes. (E) qRT‐PCR analysis of miR‐502‐3p relative expression in human cortical neurons, microglia and astrocytes (F) qRT‐PCR analysis of miR‐502‐3p relative expression in mouse hippocampal neurons, microglia and astrocytes. Relative expression of miR‐501‐3p and miR‐502‐3p were calculated using the 2−Δct method. Statistical analysis was performed using one‐way ANOVA with multiple comparisons tests. Data are presented as mean ± SEM ( n = 3).

Article Snippet: To assess the miR‐501‐3p and miR‐502‐3p expression in both human and mouse brain cell types, human cortical neurons (HCN‐2/Cat # CRL‐3592), human microglia (HMC3/Cat # CRL‐3304), human astrocytes (CCF‐STTG1/Cat # CRL‐1718), mouse microglia (SIM‐A9/Cat # CRL‐3265) and mouse astrocytes (C8‐D1A/Cat # CRL‐2541) were obtained from the American type culture collection (ATCC, Manassas, USA), and mouse hippocampal neurons (mHippoE‐14/Cat # CLU‐198), from the Cedarlane Laboratories (North Carolina, USA).

Techniques: Expressing, Derivative Assay, Microscopy, Quantitative RT-PCR